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Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae) - doi: 10.4025/actascibiolsci.v34i1.6961 Biological Sciences
Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO; Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC; Bessa, Érika Façanha; Universidade Federal do Ceará - UFC; Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC.
Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal...
Palavras-chave: 2.08.01.00-9 Halymeniales; Sulfated macromolecules; Drying methods; Blood coagulation 2.08.01.00-9.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961
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